血管平滑肌细胞增殖过程中钙调神经磷酸酶对三磷酸肌醇受体Ⅰ型表达的调节

目的研究血管平滑肌细胞(VSMCs)增殖过程中钙调神经磷酸酶(CaN)对三磷酸肌醇受体Ⅰ型(IP3RⅠ)表达的调节。方法植块法分离培养VSMCs,比色法(MTT法)测定VSMCs的增殖,荧光染色法测定VSMCs的活性,RT-PCR检测IP3RⅠ型mRNA转录水平,免疫印迹法(Westernblot)测定IP3RⅠ型蛋白质翻译水平。结果苯肾上腺素(PE)组VSMCs吸光度显著高于对照组(P<0·05),环孢素A(CsA)组和CsA PE组VSMCs吸光度显著低于对照组(P<0·05)。PE组活细胞百分数与对照组无显著差异(P>0·05),CsA组和CsA PE组活细胞百分数显著低于对照组(P<0·05)。PE组IP3RⅠ型mRNA表达水平显著高于对照组(P<0·05),CsA组和CsA PE组mRNA水平显著低于对照组(P<0·05)。PE组IP3RⅠ型蛋白质表达水平显著高于对照组(P<0·05),CsA组和CsA PE组蛋白质表达水平显著低于对照组(P<0·05)。结论CaN调节VSMCs增殖与活性,并且在转录水平和翻译水平调节IP3RⅠ的表达。     

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侵袭性真菌感染(IFI)最多发生于免疫受损或免疫功能低下的患者中,抗真菌治疗的效果直接关系到此类患者的基础疾病的疗效和长期存活,应重视抗真菌药物的规范应用。文章阐述了规范性应用抗真菌药物的概念,提倡依据不同危险因素的患者群推测可能的致病真菌,以诊断分层为基础,综合药物特性、区域性特点和价格效能比,足量、足疗程地使用抗真菌药物,并依据指南疗效评估标准评价治疗效果并调整用药。     

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急性白血病肺部并发症根据病因分为感染性及非感染性两大类。近年来,随着对感染性疾病的预防和治疗水平的提高,使得非感染性肺部并发症的诊治显得更为重要。非感染肺部并发症可发生在疾病各个阶段,尤其是异基因HSCT后所占比例更大,病因更为复杂。该文主要对急性白血病非感染性肺部并发症进行综述。     

利妥昔单抗联合CHOP方案治疗血管免疫母T细胞淋巴瘤1例报告

血管免疫母T细胞淋巴瘤(AITL)是最常见的原发于淋巴结的外周T细胞性淋巴瘤,占外周T细胞性淋巴瘤的15%～20%,呈侵袭性进展。有学者报道,利妥昔单抗联合CHOP方案治疗AITL可改善其预后[1]。本文报道1例使用利妥昔单抗联合CHOP(R-CHOP)方案治疗AITL的经     

Risk stratification-directed donor lymphocyte infusion could reduce relapse of standard-risk acute leukemia patients after allogeneic hematopoietic stem cell transplantation

We studied the impact of risk stratification-directed interventions for minimal residual disease (MRD) on relapse and disease-free survival (DFS) prospectively in 814 subjects with standard-risk acute leukemia receiving allotransplantation in first or second complete remission. A total of 709 subjects were MRD(-) after transplantation (Group A); 105 subjects were MRD(+), 49 received low-dose IL-2 (Group B), and 56 received modified donor lymphocyte infusion (DLI) with or without low-dose IL-2 (Group C). Posttransplantation immune suppression for GVHD was also modified based on MRD state. The cumulative risk of relapse was significantly less and DFS was significantly better in subjects in Group C than in subjects in Group B (P = .001 and P = .002, respectively), but was not different from subjects in Group A (P = .269 and P = .688, respectively). Multivariate analyses confirmed that MRD state and modified DLI were significantly correlated with relapse (P = .000, odds ratio [OR] = 0.255 and P = .000, OR = 0.269) and DFS (P = .001, OR = 0.511 and P = .006, OR = 0.436, respectively). These data suggest that risk stratification-directed interventions with modified DLI in patients with standard-risk acute leukemia who are MRD(+) after transplantation may improve transplantation outcomes.     

促周围神经再生中药复方——复方红芪

周围神经损伤是临床常见问题[1],尽管目前周围神经修复技术已经有了很显著的进步,但即使新鲜、清洁的周围神经断裂伤,能及时运用显微外科技术进行修复,也往往不能完全再生,患者神经功能恢复不能令人满意[2]。因此,如何促进周围神经损伤     

Measurement of cortical bone thickness in adults by cone-beam computerized tomography for orthodontic miniscrews placement

The purpose of this study was to investigate the cortical bone thickness of the inter-dental area of both jaws for orthodontic miniscrew placement. The cone-beam computerized tomography images of 32 non-orthodontic adults with normal occlusion were taken to measure the cortical bone thickness in both jaws. One-way analysis of variance (ANOVA) was used to analyze the differences in cortical bone thickness. Buccal cortical bone in the mandible was thicker than that in the maxilla. In the maxilla, cortical bone thickness was thicker in the buccal side than in the palatal side. Buccal cortical bone thickness in the mandible was thickest at the site distal to the first molar, and in the maxilla it was thickest at the site mesial to the first molar, while in the palatal side of maxilla it was thickest at the site mesial to the second premolar. The changing pattern of cortical bone thickness varies at different sites. In the buccal side of maxilla, the thinnest cortical bone thickness was found to be at 4 mm level from the alveolar crest, while the thickest was at 10 mm level (except for the site mesial to the first premolar). The buccal cortical bone thickness at the sites mesial or distal to the first molar in the mandible and palatal cortical bone thickness of maxilla tended to increase with increasing distance from the alveolar bone.     

RNA干扰COPS3基因抑制骨肉瘤细胞HOS增殖和迁移能力的实验研究

目的 探讨COPS3基因的表达对骨肉瘤细胞系HOS增殖和迁移能力的影响.方法 构建针对COPS3 mRNA的慢病毒表达载体(Lenti-COPS3si)和阴性对照载体(Lenti-NC),并将其感染高表达COPS3基因的人骨肉瘤细胞系HOS.实时定量PCR检测COPS3 mRNA的表达;Western blot测定COPS3的蛋白表达;噻唑蓝(MTT)比色法检测HOS细胞的增殖能力;迁移实验检测HOS细胞迁移能力的改变.结果 与空白对照组和转染Lenti-NC的阴性对照组相比,转染48h后Lenti-COPS3si组COPS3 mRNA和蛋白的表达均明显降低,转染48h后,Lenti-COPS3si组HOS细胞的增殖能力明显下降(P＜0.05).迁移实验结果显示,与空白对照组和阴性对照组相比,细胞的迁移能力下降(P＜0.05).结论 慢病毒载体Lenti-COPS3si可以特异性抑制骨肉瘤细胞系HOS中COPS3的表达,并显著降低细胞的增殖和迁移能力,此技术有望成为抑制骨肉瘤生长和转移的新途径.